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Characterization of patient-derived MPM cell lines. ( A ) Representative images showing different morphology of three BAP1 positive (BAP1+) and three BAP1 negative (BAP1−) MPM cell lines (scale bar = 100 µm). ( B ) Flow cytometry plot representing the percentage of pancytokeratine positive cells in the indicated MPM cell lines. ( C ) Immunohistochemical analysis of BAP1, pan-cytokeratin (pan-CK), Wilms tumor-1 antigen <t>(WT1),</t> and calretinin (CALR) in the indicated MPM cell lines (scale bar = 100 µm). ( D ) Western blot analysis showing BAP1 status of the reported MPM cell lines.
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Characterization of patient-derived MPM cell lines. ( A ) Representative images showing different morphology of three BAP1 positive (BAP1+) and three BAP1 negative (BAP1−) MPM cell lines (scale bar = 100 µm). ( B ) Flow cytometry plot representing the percentage of pancytokeratine positive cells in the indicated MPM cell lines. ( C ) Immunohistochemical analysis of BAP1, pan-cytokeratin (pan-CK), Wilms tumor-1 antigen <t>(WT1),</t> and calretinin (CALR) in the indicated MPM cell lines (scale bar = 100 µm). ( D ) Western blot analysis showing BAP1 status of the reported MPM cell lines.
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Characterization of patient-derived MPM cell lines. ( A ) Representative images showing different morphology of three BAP1 positive (BAP1+) and three BAP1 negative (BAP1−) MPM cell lines (scale bar = 100 µm). ( B ) Flow cytometry plot representing the percentage of pancytokeratine positive cells in the indicated MPM cell lines. ( C ) Immunohistochemical analysis of BAP1, pan-cytokeratin (pan-CK), Wilms tumor-1 antigen <t>(WT1),</t> and calretinin (CALR) in the indicated MPM cell lines (scale bar = 100 µm). ( D ) Western blot analysis showing BAP1 status of the reported MPM cell lines.
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Characterization of patient-derived MPM cell lines. ( A ) Representative images showing different morphology of three BAP1 positive (BAP1+) and three BAP1 negative (BAP1−) MPM cell lines (scale bar = 100 µm). ( B ) Flow cytometry plot representing the percentage of pancytokeratine positive cells in the indicated MPM cell lines. ( C ) Immunohistochemical analysis of BAP1, pan-cytokeratin (pan-CK), Wilms tumor-1 antigen <t>(WT1),</t> and calretinin (CALR) in the indicated MPM cell lines (scale bar = 100 µm). ( D ) Western blot analysis showing BAP1 status of the reported MPM cell lines.
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Characterization of patient-derived MPM cell lines. ( A ) Representative images showing different morphology of three BAP1 positive (BAP1+) and three BAP1 negative (BAP1−) MPM cell lines (scale bar = 100 µm). ( B ) Flow cytometry plot representing the percentage of pancytokeratine positive cells in the indicated MPM cell lines. ( C ) Immunohistochemical analysis of BAP1, pan-cytokeratin (pan-CK), Wilms tumor-1 antigen <t>(WT1),</t> and calretinin (CALR) in the indicated MPM cell lines (scale bar = 100 µm). ( D ) Western blot analysis showing BAP1 status of the reported MPM cell lines.
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Characterization of patient-derived MPM cell lines. ( A ) Representative images showing different morphology of three BAP1 positive (BAP1+) and three BAP1 negative (BAP1−) MPM cell lines (scale bar = 100 µm). ( B ) Flow cytometry plot representing the percentage of pancytokeratine positive cells in the indicated MPM cell lines. ( C ) Immunohistochemical analysis of BAP1, pan-cytokeratin (pan-CK), Wilms tumor-1 antigen <t>(WT1),</t> and calretinin (CALR) in the indicated MPM cell lines (scale bar = 100 µm). ( D ) Western blot analysis showing BAP1 status of the reported MPM cell lines.
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Characterization of patient-derived MPM cell lines. ( A ) Representative images showing different morphology of three BAP1 positive (BAP1+) and three BAP1 negative (BAP1−) MPM cell lines (scale bar = 100 µm). ( B ) Flow cytometry plot representing the percentage of pancytokeratine positive cells in the indicated MPM cell lines. ( C ) Immunohistochemical analysis of BAP1, pan-cytokeratin (pan-CK), Wilms tumor-1 antigen (WT1), and calretinin (CALR) in the indicated MPM cell lines (scale bar = 100 µm). ( D ) Western blot analysis showing BAP1 status of the reported MPM cell lines.

Journal: Cancers

Article Title: Evaluation of the Preclinical Efficacy of Lurbinectedin in Malignant Pleural Mesothelioma

doi: 10.3390/cancers13102332

Figure Lengend Snippet: Characterization of patient-derived MPM cell lines. ( A ) Representative images showing different morphology of three BAP1 positive (BAP1+) and three BAP1 negative (BAP1−) MPM cell lines (scale bar = 100 µm). ( B ) Flow cytometry plot representing the percentage of pancytokeratine positive cells in the indicated MPM cell lines. ( C ) Immunohistochemical analysis of BAP1, pan-cytokeratin (pan-CK), Wilms tumor-1 antigen (WT1), and calretinin (CALR) in the indicated MPM cell lines (scale bar = 100 µm). ( D ) Western blot analysis showing BAP1 status of the reported MPM cell lines.

Article Snippet: The following antibodies were used: BAP-1 (Santa-Cruz Biotechnology, Santa Cruz, CA, USA, sc-28383, 1:100); Pan-cytokeratin AE1/AE3 (Dako, Agilent, Santa Clara, CA, USA, GA053, 1:500); Wilms Tumor-1 antigen (WT1) cl.6FH2 (Thermo Fisher Scientific, Waltham, MA, USA, MA1-46028, 1:10); Calretinin (Thermo Fisher Scientific, RB-9002-R7, 1:100).

Techniques: Derivative Assay, Flow Cytometry, Immunohistochemical staining, Wilms Tumor Assay, Western Blot

Histological characterization of MPM cultures.

Journal: Cancers

Article Title: Evaluation of the Preclinical Efficacy of Lurbinectedin in Malignant Pleural Mesothelioma

doi: 10.3390/cancers13102332

Figure Lengend Snippet: Histological characterization of MPM cultures.

Article Snippet: The following antibodies were used: BAP-1 (Santa-Cruz Biotechnology, Santa Cruz, CA, USA, sc-28383, 1:100); Pan-cytokeratin AE1/AE3 (Dako, Agilent, Santa Clara, CA, USA, GA053, 1:500); Wilms Tumor-1 antigen (WT1) cl.6FH2 (Thermo Fisher Scientific, Waltham, MA, USA, MA1-46028, 1:10); Calretinin (Thermo Fisher Scientific, RB-9002-R7, 1:100).

Techniques: